Development of SYBR Green real-time PCR and nested RT-PCR for the detection of Potato Mop-top Virus (PMTV) and viral surveys in Progeny tubers derived from PMTV infected Potato tubers
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چکیده
منابع مشابه
Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
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A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies tha...
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Background and purpose: There are various methods for molecular detection of SARS-CoV2 genome among which, PCR-based methods are the most reliable for making diagnosis. The majority of approved PCR kits for detection of Coronavirus are based on TaqMan real-time PCR which is expensive due to incorporating fluorescent and quencher-harboring probe. The aim of this study was to design a simple and ...
متن کاملRT-PCR-ELOSA tests on pooled sample units for the detection of virus Y in potato tubers.
A highly sensitive RT-PCR protocol able to detect potato virus Y (PVY) in pooled sample units (tubers) was developed. PVY-specific primers selected in the coat protein gene were found to amplify a 359 bp fragment from diluted crude extract of infected tubers. For the detection of the amplification products, a colorimetric detection procedure in microtiter plates was established. The amplicons a...
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ژورنال
عنوان ژورنال: Molecular and Cellular Probes
سال: 2019
ISSN: 0890-8508
DOI: 10.1016/j.mcp.2019.101438